The aim of our experiments was to characterise and to validate the osmotic fragility test when applied to human blood samples\r\nwith no significant alterations of osmotic fragility but with a differentiating shape of the haemolysis curve. All experiments were\r\ncarried out on human erythrocytes taken fromthe Regional Centre of Blood Donation and Blood Therapy inWroclaw. The washed\r\nerythrocytes were exposed to near-infrared radiation (NIR) or ozonated, and the osmotic fragility test was applied. The osmotic\r\nfragility, calculated from the experimental haemolysis curve for the control and cells irradiated for 15 min, is the same within the\r\nempirical error. Calculation of the first derivative of the haemolysis curve allowed us to visualise the changes in osmotic fragility\r\ndistribution after exposure to NIR. By contrast, significant changes both to the osmotic fragility value and the distribution of osmotic\r\nproperties were observed after an erythrocytes ozonation procedure. Description of cell osmotic properties requires at least two\r\nparametersââ?¬â?the value of osmotic fragility and the slope of the haemolysis curve in the region where absorbance sharply increases\r\ndue to cell haemolysis.
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